P-804 Endometrial recovery via implantable CD133+ stem cells scaffold in Asherman’s syndrome therapy

Abstract Study question Can we use CD133+ bone marrow derived stem cell (BMDSCs) loaded scaffolds to aid endometrial regeneration locally in Asherman syndrome (AS)? Summary answer Gelatin supported transdifferentiation via decidualization of BMDSCs. Implantable showed a certain degree recovery an AS rat model. What is known already We have demonstrated that BMDSCs contribute proliferation, and functional murine models (Cervelló et al., 2015) humans (Santamaria 2016). This advanced therapy Phase II Clinical trial regulated by EMA. Here, bioengineered loaded-cell obtain more localized therapy. design, size, duration were prepared cryogelation analyzed micro-computed tomography. For vitro studies, cultured into the decidualized with 1 mM 8-Bromoadenosine 3´5´-cyclic monophosphate sodium salt (cAMP) for 7 days. studies vivo, used as control group (gelatin scaffold only) gelatin implanted acid-damaged horns induced model followed after one three weeks. Participants/materials, setting, methods DNA quantification was carried out viability on scaffolds, whereas immunofluorescence, single RNA sequencing RT-qPCR assess decidualization. screened scanning electron microscopy (SEM), hematoxylin eosin staining (H&E) RT-qPCR. Main results role chance Cell spreading proliferation observed due its high pore size similarity native extracellular matrix. IGFBP1 Prolactin expression determined immunofluorescence CD133 stromal decidual cells. Additionally, data revealed higher specific canonical markers (ENG, CXCL12, PDGFRB) associated genes (IRS2, COCH) scaffold. During vivo AS, studied set inflammatory paracrine secretory molecules. scaffold, obtained decrease NF-kB (p ≤ 0.01). number glands well thickness increasing trend 3 weeks implantation. Limitations, reasons caution Endometrial functionality should be further analysed needed full recovery. Wider implications findings Our mimic matrix, contributing viability, The herein open new avenues regeneration, especially patients. Trial registration Not applicable